Figure 5.

CRFR1 in AgRP Neurons Are Required for Adaptive Thermogenesis

(A) Growth curves of female and male AgRPΔCRFR1 mice and control littermates (n = 3–18).

(B) Heat production of female AgRPΔCRFR1 mice and control littermates (n = 10 and 11).

(C) Body temperature of female AgRPΔCRFR1 mice and control littermates (n = 6 and 8).

(D) Relative Pomc and Mc4r levels in hypothalami of female AgRPΔCRFR1 mice and control littermates (n = 8–12).

(E) Body temperature during cold challenge test (n = 6 and 8).

(F and G) Relative BAT Ucp1 expression (F) (n = 6 and 7) and BAT UCP1 immunoreactivity (G) following 7 h at 5°C.

(H) Relative expression of thermogenic-related genes in inguinal WAT following 7 h at 5°C (n = 5–8).

Data are shown as mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01. See also Figure S6.