Skip to main content
. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: Dev Cell. 2016 Apr 21;37(3):279–288. doi: 10.1016/j.devcel.2016.03.025

Figure 4. The ER stress response and ER inheritance are defective in rtn1Δrtn2Δyop1Δ cells and this is rescued by deletion of Lnp1.

Figure 4

(A–J) FRAP analysis of the pnER and cER of Kar2-sfGFP-expressing WT (A and F), slt2Δ (B and G), rtn1Δrtn2Δyop1Δ (C and H), rtn1Δrtn2Δyop1Δsey1Δ (D and I), and rtn1Δrtn2Δyop1Δlnp1Δ cells (E and J). Graphs are the mean ± SD of 3 experiments, each examining ≥7 cells.

(K–O) Quantification of cER inheritance in WT (K), rtn1Δrtn2Δyop1Δ (L), slt2Δ (M), rtn1Δrtn2Δyop1Δsey1Δ (N), and rtn1Δrtn2Δyop1Δlnp1Δ cells (O) expressing Hmg1-GFP. Cells were treated with DMSO (dark blue) or Tm (pale blue) and cER inheritance was scored as described in Figure 3I–J. Graphs show the mean ± SD of 3 experiments, each with ≥200 cells counted.

(P) Quantitation of early ER tubule formation in WT, slt2Δ, rtn1Δrtn2Δyop1Δ, rtn1Δrtn2Δyop1Δlnp1Δ, and rtn1Δrtn2Δyop1Δsey1Δ cells expressing Hmg1-GFP. Cells were treated with DMSO or Tm for 3 hr. Quantitations are the mean ± SD of 3 experiments, each analyzing ≥40 cells.

(Q) Correlation of the block in initial tubule formation [100 – 100 × (% tubule formation with Tm)/(% tubule formation with DMSO)] (Figure 4K–4O) and cER inheritance [100 – 100 × (% cER inheritance with Tm)/(% cER inheritance with DMSO)] (Figure 4P) for WT, slt2Δ, rtn1Δrtn2Δyop1Δ, rtn1Δrtn2Δyop1Δlnp1Δ, and rtn1Δrtn2Δyop1Δsey1Δ cells expressing Hmg1-GFP. The data represent the mean ± SD of 3 experiments. See also Figures S3 and S4.