Figure 5.

Phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic relationship between members of the phylum Chloroflexi (A) and Bacteroidetes-Chlorobi (B) phyla and sequences obtained from the Mushroom Spring microbial undermat community. The tree was generated based on the Maximum Likelihood method using the phyML software included in the ARB package. Percentage numbers on nodes refer to 100 bootstrap pseudoreplicates conducted. Only values >50% are shown. Bold sequences were obtained from Mushroom or Octopus Spring in this or previous studies. Red bold labels indicate sequences obtained in this study. Blue bold labels indicate “OS type” sequences from previous studies. OTU numbers shown refer to the most abundant OTU represented by the sequence. Only sequences with length >1,000 bp were used for phylogenetic calculations. Sequence length <1,000 bp are given in (gray) in the labels and corresponding sequences were added using the Parsimony method without changing tree topology.