Table 1.
Most abundant OTUs (97% nt identity), number of reads and relative abundance, microdiversity in terms of represented dereplicated iTag sequences, corresponding metagenome sequences and next relatives determined by BLAST search.
| OTU-name | Reads | Rel. abundance (%) | No. of derep. iTags | Metagenome 16S rRNA | Relevant Blast hits | Acc. No. | Ident (%) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Total | >100 | Scaffold ID | Avg. coverage | Length (bp) | ||||||
| OTU-01 | 68,369 | 49 | 6,193 | 24 | 101,6681 | 2,842 | 1,357 | Roseiflexus sp. RS-1 | NR_074197 | 100 |
| OTU-02 | 14,480 | 10 | 1,664 | 3 | 1,000,336 | 458 | 1,426 | Thermotoga hypogea NBRC 106472 (T) | AP014508 | 98 |
| OTU-03 | 6,203 | 4 | 2,082 | 9 | 1,062,246 | 951 | 710 | Unc. bacterium clone SM2D03 | AF445720 | 100 |
| Eubacterium sp. (OS type L) | L04707 | 98 | ||||||||
| OTU-04 | 6,160 | 4 | 899 | 3 | 1,002,657 | 262 | 1,434 | clone YNP_SBC_BP2A_B2 | HM448202 | 99 |
| Thermocrinis ruber strain DSM 23557(T) | CP007028 | 97 | ||||||||
| OTU-05 | 5,046 | 4 | 1,289 | 7 | 1,008,881 | 105 | 1,002 | Synechococcus sp. JA-2-3B'a(2-13) | CP000240 | 100 |
| OTU-06 | 3,580 | 3 | 675 | 1 | 1,001,696 | 423 | 1,364 | clone YNP_SBC_BP4_B2 | HM448255 | 98 |
| Thermanaerothrix daxensis GNS-1 (T) | HM596746 | 87 | ||||||||
| OTU-07 | 3,350 | 2 | 564 | 1 | 1,014,288 | 189 | 1,444 | OP9 bacterium clone TP29 | EF205555 | 98 |
| Ammonifex thiophilus strain SR(T) | EF554597 | 83 | ||||||||
| OTU-08 | 3,283 | 2 | 705 | 2 | 1,000,748 | 135 | 1,413 | clone SMD-B01 | AB477993 | 99 |
| Thermodesulfovibrio yellowstonii DSM11347(T) | NR_074345 | 96 | ||||||||
| OTU-09 | 1,981 | 1 | 449 | 2 | 1,000,273 | 129 | 1,389 | clone NY-30 | KC290430 | 94 |
| Thermanaerothrix daxensis GNS-1 (T) | HM596746 | 91 | ||||||||
| OTU-10 | 1,715 | 1 | 510 | 6 | 1,001,962 | 67 | 1,406 | EM3 clone OPB88 | AF027006 | 99 |
| Rhodothermus marinus SG0.5JP17-172 (T) | CP003029 | 82 | ||||||||
| OTU-11 | 1,594 | 1 | 521 | 3 | 1,030,146 | 83 | 1,309 | Chloroflexus sp. MS-G | KR230107 | 99 |
| Chloroflexus aurantiacus J-10-fl (T) | CP000909 | 95 | ||||||||
| OTU-12 | 1,569 | 1 | 477 | 3 | 1,003,586 | 802 | 1,415 | OP10 clone OPB80 | AF027089 | 94 |
| Fimbriimonas ginsengisoli Gsoil 348 (T) | CP007139 | 81 | ||||||||
| OTU-13 | 1,392 | 1 | 306 | 2 | 1,015,572 | 36 | 1,497 | clone bac67 | HM184963 | 95 |
| Thermoanaerobaculum aquaticum MP-01 (T) | NR_109681 | 88 | ||||||||
| OTU-14 | 1,260 | 1 | 322 | 3 | 1,003,293 | 60 | 1,420 | clone TP5 | EF205581 | 99 |
| Thermogutta terrifontis R1 (T) | KC867694 | 90 | ||||||||
| OTU-15 | 1,008 | 1 | 391 | 3 | 1,021,867 | 132 | 1,299 | clone OB17 | EF429491 | 98 |
| Chloroflexus aurantiacus J-10-fl(T) | CP000909 | 90 | ||||||||