Table 5.
Overview of community composition detected by the different methods used in this study (iTag, metagenome 16S rRNA, metagenome binning) and relative abundances in undermat and upper layer iTag sequencing study.
| iTag OTU | Identity | Undermat metagenome 16S rRNA | Undermat metagenomic bin | iTag MS undermat (%) | iTag MS upper green layer (%) | Upper green layer metagenomic bina |
|---|---|---|---|---|---|---|
| OTU-01 | Chloroflexi, Roseiflexus sp., OS Type C | Yes, scaffold 166812 | Yes, bin-1 [very small] | 49.1 | 33.9 | Yes |
| OTU-02 | Thermotogae, Pseudothermotoga sp. | Yes, scaffold 336 | Yes, bin-2 | 10.4 | 1.5 | No |
| OTU-03 | OP10/Armatimonadetes_Gp7, OS Type L | Yes, scaffold 622461 | No [enrichment bin-3] | 4.5 | 0.8 | Yes |
| OTU-04 | Aquificae, Thermocrinis sp. | Yes, scaffold 2657 | Yes, bin-4 | 4.4 | 0.3 | No |
| OTU-05 | Cyanobacteria, Synechococcus sp. B', OS Type B | Yes, scaffold 8881 | Yes, bin-5 | 3.6 | 37.4 | Yes |
| OTU-06 | Chloroflexi, Anaerolineae-like' phototroph | Yes, scaffold 1696 | Yes, bin-6 | 2.6 | 1.2 | Yes |
| OTU-07 | OP9/Atribacteria | Yes, scaffold 14288 | Yes, bin-7 | 2.4 | 0.7 | No |
| OTU-08 | Nitrospirae, Thermodesulfovibrio sp. | Yes, scaffold 00748 | Yes, bin-8 | 2.4 | 2.7 | (Yesb) |
| OTU-09 | Chloroflexi, Anerolineae | Yes, scaffold 273 | Yes, bin-9 | 1.4 | 0.7 | No |
| OTU-10 | Thermotogae, EM3/OPS-2 | Yes, scaffold 1962 | Yes, bin-10 | 1.2 | 3.1 | Yes |
| OTU-11 | Chloroflexus sp. MS-G | Yes, scaffold 301461 | Yes, bin-11 | 1.1 | 1.2 | Yes |
| OTU-12 | OP10/Armatimonadetes_Gp6 | Yes, scaffold 3586 | Yes, bin-12 | 1.1 | 0.5 | No |
| OTU-13 | OP-8/Aminicenantes | Yes, scaffold 32931 | Yes, bin-13 | 1.0 | 0.0 | No |
| OTU-14 | Planctomycetes | Yes, scaffold 3293 | Yes, bin-14 | 0.9 | 0.0 | No |
| OTU-15 | Chloroflexi | Yes, scaffold 218671 | Yes, bin-15 | 0.7 | 0.0 | No |
| OTU-16 | Planctomycetes, Gemmata | Yes, scaffold 261011 | Maybe, bin-23 | 0.7 | 0.2 | No |
| OTU-17 | Chloracidobacterium thermophilum | Yes, scaffold 32584 | Yes, bin-16 | 0.7 | 5.2 | No |
| OTU-18 | Armatimonadetes_Gp 2 | Yes, scaffold 584 | Yes, bin-17 | 0.6 | 0.0 | No |
| OTU-19 | Planctomycetes, uncultured | Yes, scaffold 11289 | No | 0.6 | 0.0 | No |
| OTU-20 | Elusimicrobia, uncultured | Yes, scaffold 20130 | No | 0.5 | 0.1 | Maybe |
| OTU-21 | Deinococcus-Thermus, Meiothermus sp. | Yes, scaffolds 8341 and 25957 | Yes, bin-18 | 0.5 | 0.4 | No |
| OTU-22 | Cyanobacteria, Synechococcus sp. A, OS Type A | Yes, scaffold 85825 | No | 0.5 | 0.5 | No |
| OTU-23 | Chloroflexi, Anaerolineae, OPB12 | Yes, scaffold 18877 | No | 0.5 | 0.0 | No |
| OTU-24 | Chlorobi, Lineage 5: OPB56 | Yes, scaffold 2638 | Yes, bin-19 | 0.5 | 2.0 | No |
| OTU-25 | Spirochaetae, Leptospiraceae | Yes, scaffold 4665 | No | 0.4 | 0.1 | No |
| OTU-26 | Thermodesulfobacteria, Caldimicrobium | Yes, scaffold 193547 | Yes, bin-32 | 0.4 | 0.4 | No |
| OTU-27 | Chlorobi, Lineage 5: OPB56 | Yes, scaffold 104947 | No | 0.4 | 1.0 | No |
| OTU-28 | Thermotogae, EM3 | Yes, scaffold 49539 | No | 0.4 | 0.0 | No |
| OTU-29 | Chlorobi, Lineage 5: OPB56 | Yes, scaffold 6663 | No | 0.4 | 0.3 | No |
| OTU-30 | Thermotogae, EM3 | Yes, scaffold 26263 | No | 0.4 | 0.0 | No |
| OTU-31 | Chloroflexi, Bellilinea | Yes, scafffold 10483 | Yes, bin-25 | 0.3 | 0.1 | No |
| OTU-32 | Spirochaetae, Exilispira | Yes, scaffolds 5322 and 20220 | No | 0.2 | 1.1 | No |
| OTU-33 | OP10/Armatimonadetes_Gp7, OS Type L | Yes, scaffold 62246 | No | 0.2 | 0.0 | No |
| OTU-34 | Chlorobi, Ignavibacteriaceae | Yes, scaffolds 10292 and 68906 | Maybe, bin-24 | 0.2 | 0.2 | No |
| OTU-35 | Spirochaetae, Brevinema | No | No | 0.2 | 0.3 | No |
| OTU-36 | Acidobacterium, OPB3 | Yes, scaffolds 1343 and 2292 | Yes, bin-20 | 0.2 | 0.0 | No |
| OTU-37 | Verrucomicrobia, uncultured | Yes, scaffold 25955 | No | 0.2 | 0.0 | No |
| OTU-38 | Chlorobi, “Ca. Thermochlorobacter aerophilum” | Yes, scaffold 761 | Yes, bin-21 | 0.2 | 2.2 | No |
| OTU-39 | Chloroflexi, Anaerolineae | Yes, scaffold 145123 | No | 0.2 | 0.1 | No |
| OTU-40 | Deltaproteobacteria, Desulfarculales, uncultured | Yes, scaffold 112567 | No | 0.2 | 0.0 | No |
| OTU-41 | Spirochaetae, Spirochaeta | Yes, scaffold 19076 | No | 0.2 | 0.1 | No |
| OTU-42 | Acetothermia, uncultured | (Yes, scaffold 169700)* | No | 0.1 | 0.0 | No |
| OTU-43 | Spirochaetae, Leptospiraceae, uncultured | (Yes, scaffold 205406)* | No | 0.1 | 0.0 | No |
| OTU-44 | Deltaproteobacteria, Synthroporhabdus-like | Yes, scaffolds 233149 and 241554 | No | 0.1 | 0.0 | No |
| OTU-45 | Chlorobi, Ignavibacteriaceae | Yes, scaffolds 104127 and 141772 | Maybe, bin-24 | 0.1 | 0.0 | No |
| OTU-46 | Elioraea sp. | Yes, scaffold 23894 | Yes, bin-22 | 0.1 | 0.0 | No |
*
based on phylogenetic analysis, no overlap of sequences.
a
Klatt et al. (2011).
b
no metagenomic bin, but related sequences recruited by reference genomes.