Figure 1.

Metabolic network modeling strategy. Flow chart of analysis procedure to obtain a metabolic network which can be used for the prediction of flux and elementary modes in context with the help of various different types of data. Two steps are combined: Elementary mode analysis gives an overview on all metabolic pathways accessible for the pathogen. Based on this network, algorithms such as YANA, YANAsquare, and YANAvergence offer different routines to calculate implied flux value for different pathways using gene expression data, protein expression data or metabolite concentration changes. Targets which are critical in a metabolic sense are easily identified considering hub enzymes, enzymes with a high metabolic control and observation of flux change in general.