Table 1.

Top Targets from metabolic modeling to target pathogen metabolism^a.

Pathway		Target by pace maker enzyme	Validated by flux analysis
Vitamin	Riboflavin	RIB1	Yes
Nucleotide	Pyrimidin	Carbamoyl synthetase	Yes
Lipid	Ergosterol and	Ergosterol synthetase	Yes
	Cell wall synthesis		Yes
Carbohydrates	Glycolysis	Aldolase, enolase	Yes
	Pentose Phosphate Cylce	Transketolase	Yes

^aSummary of pathways and their primary metabolites investigated as well as important enzymes which posses a capability as pace maker in their pathway (middle column) and whether they could be validated by flux analysis, and are therefore considered as prioritized targest. Furthermore, there is evidence from other systems, e.g., overexpression of the first gene of the riboflavin biosynthetic pathway (RIB1) is already sufficient to obtain yellow colonies and the accumulation of riboflavin in the supernatant in Pichia pastoris (Marx et al., 2008). Validation by flux analysis is given in detail in Supplementary Material (Supplementary Tables 2, 4) indicating that the pathway did undergo expression changes comparing the different data sets against each other in the flux calculations.