Table 2.
Primers used for qPCR analysis of microarray-identified transcripts responsive to camelina meal-containing diets in Atlantic salmon
| Gene name (symbol) | Primer sequence (5′–3′) | Sequence used for primer design | Amplification efficiency (%) | Amplicon size (bp) |
|
|---|---|---|---|---|---|
| ES1 protein homolog (es1) | F | ACTATAAAGGGAACAGGGTGCC | EB085110 | 89.3 | 167 |
| R | CAAGCTGGTCACCTCCTCTG | ||||
| transmembrane protease serine 9-like (tmprss9; alias: polyserase-I) | F | TAGTGCCGTTAGTGCTGGTG | EG876684 | 95.8 | 194 |
| R | CCAGACCTCTGTGGTGGAAT | ||||
| thioredoxin (txn) | F | GGATTCCTTCTTCAGTGCCC | BT049355 | 90.1 | 196 |
| R | GATGTCACAGTGTTTGGCCA | ||||
| pirin (pir; alias: probable quercetin 2,3-dioxygenase) | F | GAGGTCTAAGATCAGGGATTCC | NM_001141576 | 102.6 | 200 |
| R | GGAGGCACAGTGACAAAACA | ||||
| ependymin (epd) | F | TGACTGGAGCCATGTCAGTG | BT058166 | 99.4 | 125 |
| R | CAGGCCGAATGTCTTGTTCT | ||||
| N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (napepld) | F | AAGACATCCAGGCCAGACAC | DY730770 | 99.9 | 126 |
| R | GCTCCACATTCAACCCATTC | ||||
| gamma-interferon-inducible lysosomal thiol reductase (GILT) | F | AAATGGGGAGCACACAGAAG | BT047373 | 100.2 | 105 |
| R | GCCGTACTACAGGCTTCAGG | ||||
| matrix metalloproteinase 13 (mmp13) | F | ACGATGACGAGTCCTTCAGC | DW539943 | 102.2 | 153 |
| R | AGGTGCTGGGGTTTGTGTAG | ||||
| transforming growth factor β (tgfb) | F | AGTTGCCTTGTGATTGTGGGA | EU082211 | 108.0 | 191 |
| R | CTCTTCAGTAGTGGTTTGTCG | ||||
| elongation factor 1 alpha-2 (ef1a2; normalizer) | F | TCGAGTGAGCGCACAGTAAC | GO064943 | 103.6 | 123 |
| R | CCATCTCAGCTGCTTCCTTC | ||||
| 60S ribosomal subunit L32 (rpl32; normalizer) | F | AGGCGGTTTAAGGGTCAGAT | BT043656 | 102.4 | 119 |
| R | TCGAGCTCCTTGATGTTGTG |
Primers were quality checked using the reference cDNA as the template (see Materials and Methods for details)