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. 2016 Jun 17;7:955. doi: 10.3389/fmicb.2016.00955

FIGURE 3.

FIGURE 3

Extracellular and intracellular localization of IL-36γ in epithelial cells of the human female reproductive tract following treatment with microbial products. Three-D vaginal and endocervical EC were treated with PBS, flagellin (FLA; 5 or 0.5 μg ml-1), FSL-1 (0.1 or 0.01 μg ml-1), or poly (I:C; 100, 25, or 1 μg ml-1) for 24 h. (A) Cell culture lysates and (B) supernatants were assayed by ELISA to quantify IL-36γ production and secretion. Levels of IL-36γ were determined and reported as fold change relative to PBS treated samples. Horizontal dashed line indicates IL-36γ expression in PBS treated cells. Data are shown as mean ± SD from three independent experiments each performed in duplicate. ˆP < 0.05; ∗P < 0.01; ∗∗P < 0.001; unpaired 2-tailed Student t-test with Welch’s correction.