FIGURE 5.

Recombinant IL-36γ treatment induces antimicrobial peptide expression in 3-D human vaginal and endocervical epithelial cells. 3-D vaginal and endocervical cell cultures were treated with increasing doses of recombinant IL-36γ (1, 10, 100, or 500 ng/ml) for 24 h. Relative expression levels of (A) IL-8, (B) CCL20, (C) HE4, (D) SLPI, (E) HBD-2, and (F) HBD-4 were determined by qRT-PCR and reported relative to GAPDH as fold change compared to PBS treated cells Data are shown as mean ± SD from three independent experiments each performed in duplicate. ˆP < 0.05; ^∗P < 0.01; ^∗∗P < 0.001; unpaired two-tailed Student t-test with Welch’s correction.