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. 2016 Jun 17;7:863. doi: 10.3389/fpls.2016.00863

FIGURE 4.

FIGURE 4

Phosphorylation of AtDOF4.7 by activated MPK3 and MPK6 in vitro. (A) Partial sequences of the AtDOF4.7 protein. Black boxes indicate the conserved MAPK phosphorylation sites; (B) MPK3 and MPK6 phosphorylate the MBP-tagged AtDOF4.7 recombinant protein in vitro. The arrowhead indicates the phosphorylated AtDOF4.7 protein. Bands indicated by the arrows are MPK3 or MPK6 phosphorylated by MKK5DD. Anti-His and anti-MBP tag primary antibodies were used to detect the corresponding proteins in the phosphorylation buffer.