Table 2.
The stability and precision of fluorescent microplate reader assay.
| Stains | Time (h) | The counts of Fluorescent microplate reader (mean±SD) | ||||
|---|---|---|---|---|---|---|
| Blank control | Positive control | INH (RFR*) | RIF (RFR*) | STR (RFR*) | ||
| H37Rv | 0 | 5476.00 ± 342.39 | 9902.00 ± 503.95 | 6926.00 ± 84.44 (32.76)* | 6296.00 ± 267.82 (18.53) | 6540.00 ± 256.12 (24.04) |
| 4 | 5336.00 ± 359.35 | 9626.00 ± 515.10 | 6792.00 ± 181.30 (33.94) | 6974.00 ± 323.77 (26.53) | 6766.00 ± 253.24 (33.33) | |
| 24 | 5218.00 ± 299.03 | 10088.00 ± 624.40 | 6414.00 ± 283.16 (24.60) | 6364.00 ± 335.01 (23.53) | 6476.00 ± 302.62 (25.83) | |
| 14031 | 0 | 5144.00 ± 184.07 | 8650.00 ± 347.20 | 8058.00 ± 297.19 (83.11) | 8688.00 ± 413.55 (108.08) | 8314.00 ± 533.32 (90.42) |
| 4 | 5140.00 ± 252.59 | 8410.00 ± 312.17 | 7992.00 ± 342.15 (87.22) | 8302.00 ± 452.46 (97.70) | 8352.00 ± 448.74 (98.23) | |
| 24 | 5318.00 ± 332.60 | 8558.00 ± 257.04 | 8230.00 ± 278.57 (89.88) | 8470.00 ± 527.4 (97.28) | 8516.00 ± 310.85 (98.70) | |
| 14161 | 0 | 4568.00 ± 273.17 | 8354.00 ± 417.17 | 7694.00 ± 327.46 (82.57) | 5380.00 ± 185.07 (21.45) | 7612.00 ± 353.09 (80.40) |
| 4 | 4576.00 ± 298.63 | 8338.00 ± 363.00 | 7566.00 ± 458.34 (79.48) | 5768.00 ± 206.57 (31.69) | 7650.00 ± 401.25 (81.71) | |
| 24 | 4688.00 ± 80.44 | 8502.00 ± 378.31 | 7948.00 ± 382.84 (85.47) | 5320.00 ± 257.97 (16.57) | 7884.00 ± 477.94 (83.80) | |
*
Remaining fluorescence rate (RFR) = (reaction -blank)/(positive control–blank) × 100% (“reaction” indicates the well with drug exposure; “blank” indicates the well without drug and phage; “positive control” indicates the reaction without drug).