Skip to main content
. 2016 May 12;6(6):985–992. doi: 10.1016/j.stemcr.2016.04.010

Figure 1.

Figure 1

The Current B6.SJL Strain Shows an Inherent Competitive Disadvantage

(A) A model of a typical competitive bone marrow transplantation experiment. In the experiment, bone marrow cells from a TEST (CD45.2) mouse are combined with an equal number of bone marrow cells from a COMPETITOR (CD45.1) mouse and transplanted into irradiated recipient mice. The peripheral blood chimerism is followed for 16–20 weeks as a functional assay of hematopoietic stem cell fitness.

(B) HSPCs derived from the current B6.SJL (CD45.1) competitor strain show an inherent competitive disadvantage when compared with HSPCs from wild-type C57BL/6 mice. In these experiments, the bone marrow from three littermate C57BL/6 mice or three littermate B6.SJL mice were pooled. 500,000 nucleated bone marrow cells from each donor strain were combined (1 million cells total) and transplanted by intravenous injection in lethally irradiated recipients. Four experiments were performed, two in competition with WT C57BL/6J donors and two in competition with WT C57BL/6NJ donors. Results represent the mean ± SEM. Given the large number of replicates (74 recipient mice in each arm), the error bars are not visible as they are smaller than the squares. ∗∗∗p < 0.001.