Figure 5.

Loss of MYD88 but Not TRIF Preserves Myeloid Differentiation

(A) Purified Lin⁻ cells were cultured with PBS or LPS in vitro and analyzed 12 hr later. Bar graphs show the percentage of LSK, MP (CMP + GMP) in Lin⁻ cells, and GR1⁺MAC1⁺ in total alive cells (n = 6).

(B and C) CMP and GMP subsets were sorted from WT, TRIF^−/−, and MYD88^−/− mice exposed to LPS or PBS for 24 hr. Sorted cells were cultured in vitro in myeloid differentiation conditions. Representative contour plots (left) and average percentage (right) of GR1⁺MAC1⁺ cells generated from CMPs at day 4 (B) and from GMPs at day 2 (C) (n = 3–4).

Data represent mean ± SEM. LPS versus PBS: ^∗p < 0.05, ^∗∗p < 0.01; MYD88^−/− versus WT or TRIF^−/−: ˆˆp < 0.01.