Table 3. Mean Ct end-point fluorescence of 10-fold serial dilutions of W. cibaria cloned DNA, genomic DNA and a cell suspension, as determined using real-time PCR.
| Cloned DNA |
Genomic DNA |
Cell suspension |
|||
|---|---|---|---|---|---|
| Weight/μl reaction mix | Ct ± SD (n = 3) | Weight/μl reaction mix | Ct ± SD (n = 3) | CFU/ml reaction mix | Ct ± SD (n = 3) |
| 5 ng (1.44 × 109 copies) | 13.46 ± 0.19 | N.D.a | N.D. | N.D. | N.D. |
| 500 pg (1.44 × 108 copies) | 16.58 ± 0.07 | 5 ng | 17.29 ± 0.14 | N.D. | N.D. |
| 50 pg (1.44 × 107 copies) | 20.08 ± 0.18 | 500 pg | 20.53 ± 0.04 | N.D. | N.D. |
| 5 pg (1.44 × 106 copies) | 23.45 ± 0.23 | 50 pg | 24.07 ± 0.11 | 1.95 × 108 | 24.36 ± 0.54 |
| 500 fg (1.44 × 105 copies) | 26.62 ± 0.20 | 5 pg | 27.39 ± 0.08 | 1.95 × 107 | 27.87 ± 0.16 |
| 50 fg (1.44 × 104 copies) | 29.93 ± 0.16 | 500 fg | 30.45 ± 0.07 | 1.95 × 106 | 30.97 ± 0.22 |
| 5 fg (1.44 × 103 copies) | 32.95 ± 0.46 | 50 fg | 34.96 ± 1.16 | 1.95 × 105 | 32.79 ± 0.22 |
aN.D., Not determined.