Figure 6. Size and size distribution of the MS2(9x) silk spheres.

Spheres were prepared by mixing: (A,B) silk protein (at an initial concentration of 2.5 mg/ml) with various potassium phosphate concentrations at pH 8 and (C,D) silk protein at various concentrations with 2 M potassium phosphate at pH 8. (A,C) Size of spheres, error bars show the standard deviation from the mean diameter of the spheres from three independent experiments, ***indicates statistical significance with p < 0.001, **p < 0.01, and *p < 0.05. (B,D) Size distribution of spheres, whiskers show the minimal and maximal sphere size, the bottom and the top of the box show the firs and the third quartile, respectively. The line inside the box shows median size. The data are presented from one representative experiment.