Figure 5. IFNγ secretion of PbA peptide stimulated splenocytes and CD8⁺ T cells of PbA-infected mice.

Wt and Il22^−/− mice were infected with 1 × 10⁵ PbA iRBC. At d6 p.i. mice were sacrificed and the splenocytes were stimulated with 1 μg/mL PbA peptide for 24 h. The IFNγ concentration in the supernatant was analysed with ELISA (n_{wt; w/o} = 4; _{; w/o} = 4; n_{wt n.inf; PbA Peptide} = 4; n_{wt; PbA Peptide} = 4; n_{; PbA Peptide} = 4) (A). Wt and Il22^−/− mice were infected with 1 × 10⁵PbA iRBC. At d6 p.i. mice were sacrificed and splenic CD8⁺ T cells were purified. CD8⁺ T cells of Il22^−/− or wt mice were subsequently incubated on previously PbA peptide pulsed Il22^−/− or wt BMDCs. After an incubation time of 24 h the IFNγ concentration in the supernatant was analysed with an ELISA (n_{wt CD8+; w/o PbA Peptide; wt BMDC} = 8; n_{wt CD8+; PbA Peptide; wt BMDC} = 6; _{CD8+; PbA Peptide; wt-BMDC} = 6; n_{wt CD8+; PbA Peptide; Il22}^−/− _BMDC = 8; _{CD8+; PbA Peptide; Il22}^−/− _BMDC = 8) (B). Depicted are the means ± SEM.