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. 2016 Jun 17;6:28217. doi: 10.1038/srep28217

Figure 4. Homologous recombination proficiency in cell lines used in the study.

Figure 4

Cells grown on coverslips were irradiated with 5 Gy and formation of nuclear DNA repair foci was evaluated after 6 hours using immunofluorescence. All BRCA1-wild-type cell lines readily form RAD51 foci colocalizing with γH2AX foci or BRCA1 foci colocalizing with 53BP1 foci indicating functional homologous recombination. Notice that SUM149PT cells also form nuclear foci despite mutant BRCA1. Scale bars represent 20 μm.