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. 2016 Jun 17;6:28225. doi: 10.1038/srep28225

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The full-length of 193 CIPK protein sequences from soybean, grape, Arabidopsis, rice, amborella, ginkgo, pine, gnetum, ephedra, welwitschia, fern, spikemoss, moss and green algae were used to construct the phylogenetic tree using MEGA with the Neighbor-joining (NJ) method. Bootstrap values (on nodes) were calculated using 1000 replicates. Subfamilies are highlighted with different colors. The CIPK proteins in soybean were marked by green dots. A green algae CIPK protein was used as an outgroup and marked with a red triangle. Eudicot CIPK proteins were marked with dots. Monocotyledons were marked with squares.