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. 2016 Jun 17;6:28178. doi: 10.1038/srep28178

Figure 1. Albumin and urea secretion in MPCCs treated with varying levels of glucose.

Figure 1

Fluctuations in culture medium glucose concentration in hypo- (A), normo- (B) and hyperglycemic (C) MPCCs over 3 weeks of culture. All cultures were initially cultured in a normoglycemic (5 mM glucose) culture medium for 4 days and then switched to their respective glycemic conditions (0.4–0.5 mM glucose for hypoglycemic and 25 mM glucose for hyperglycemic). Glucose levels in the depleted culture medium were measured with every 2-day culture media exchange. Dashed lines indicate average depleted glucose levels. Levels of albumin (ALB), arginase 1 (ARG1) and hepatocyte nuclear factor 4 α (HNF4A) mRNA transcripts (D) in MPCCs treated with hypo- and hyperglycemic culture media for either 10 or 18 days. Data is normalized to a normoglycemic control. Albumin (E) and urea secretion (F) in MPCCs incubated for up to 18 days in the varying glucose levels. Error bars represent SD. *p ≤ 0.05 and **p ≤ 0.01. Trends were observed in multiple hepatocyte donors.