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. 2016 Jun 17;6:28354. doi: 10.1038/srep28354

Figure 2. Effects of flounder miRNAs on megalocytivirus RBIV-C1 replication.

Figure 2

(A) Flounders were infected with megalocytivirus in the presence or absence (control) of the agomirs of 8 flounder miRNAs, and viral copies in the spleen were determined at various time points. (B) Flounders were infected with megalocytivirus in the presence or absence (control) of pol-miR-731 agomir, pol-miR-731 antagomir, agomir negative control, or antagomir negative control, and viral copies in the spleen were determined at 3, 4band 5 days post-infection (dpi). (C) Flounders were infected with megalocytivirus and pol-miR-731expression and viral numbers in spleen were determined at different dpi. (D) Positive correlations between viral replication and pol-miR-731 expression level were determined at 3 d post-viral infection (dpi) (green spots) and 4 dpi (red spots). All experiments were performed three times, and values are shown as means ± SEM. *P < 0.05, **P < 0.01.