Skip to main content
. 2016 Jun 17;6:28354. doi: 10.1038/srep28354

Figure 3. Effect of pol-miR-731 on expression of putative target genes.

Figure 3

(A) Flounders were infected with megalocytivirus RBIV-C1 in the presence of pol-miR-731agomir, pol-miR-731 antagomir, agomir negative control, or antagomir negative control, and the expression levels of 11 potential target genes in spleen were determined by q RT-PCR. (B) Flounders were infected with megalocytivirus as in (A), and proteins prepared from the spleen were analyzed by immunoblot with antibodies against recombinant IRF7, p53, or EF1A. (C) 293T cells were co-transfected with pol-miR-731 mimic, pol-miR-731mimic-Mut, and different reporter plasmids (pMIR-IRF7 3′-UTR, pMIR-p53 3′-UTR, pMIR-IRF7 3′-UTR-Mut, and pMIR-p53 3′-UTR-Mut). Relative luciferase activity was measured after 24 h of transfection. The experiment was performed three times, and values are shown as means ± SEM.*P < 0.05, **P < 0.01. (D) Schematic diagrams showing pol-miR-731 target sites on the 3′-UTR regions of IRF7 and p53. (E,F) Flounders were infected with megalocytivirus, and correlations between mRNA levels of pol-miR-731 and IRF7 (E) and p53 (F) were examined by Spearman analysis. Values of correlation coefficient (r) and P are shown.