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. 2016 Jun 17;6:28354. doi: 10.1038/srep28354

Figure 5. Effect of pol-miR-731 on cell apoptosis.

Figure 5

(A) Flounders were infected with megalocytivirus RBIV-C1, and splenocytes apoptosis was determined by Annexin V-PI assay at various days post-infection (dpi) (upper panel). Lower panel shows the results of one representative experiment. (B,C) Flounders were infected with megalocytivirus in the presence or absence (control) of pol-miR-731agomir, pol-miR-731antagomir, agomir negative control, or antagomir negative control, and splenocyte apoptosis was assayed at 4 dpi using Annexin V-PI (B). The results of one representative experiment are shown in (C). (D) Flounders were infected with megalocytivirus as in (A), and expression levels of Bax were determined by qRT-PCR. (E) FG-9307 cells were transfected with or without (control) pol-miR-731 mimic, mimic negative control, pol-miR-731 mimic plus pCNp53, or pol-miR-731 mimic plus pCN3, treated with 1 μM doxycycline, and Bax expression was determined as above. All experiments were performed three times, and values are shown as means ± SEM.*P < 0.05, **P < 0.01.