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. 2016 Jun 17;6:28354. doi: 10.1038/srep28354

Figure 6. Effect of pol-miR-731 on cell cycle arrest.

Figure 6

(A) Flounders were infected with megalocytivirus RBIV-C1, and splenocytes were examined for cell cycle arrest at various days post-infection (dpi) (upper panel). Lower panel shows the results of one representative experiment. (B,C) Flounders were infected with megalocytivirus in the presence or absence (control) of pol-miR-731agomir, pol-miR-731antagomir, agomir negative control, or antagomir negative control, and cell cycle arrest in splenocytes was assayed at 4 dpi by flow cytometry (B). The results of one representative experiment are shown in (C). (D) Flounders were infected with megalocytivirus as in (B), and expression levels of p21 were determined by qRT-PCR. (E) FG-9307 cells were transfected with or without (control) pol-miR-731 mimic, mimic negative control, pol-miR-731 mimic plus pCNp53, or pol-miR-731 mimic plus pCN3, treated with 1 μM doxycycline, and p21 expression levels in the transfectants were determined as above. All experiments were performed three times, and significant differences in values are indicated by asterisks. *P < 0.05, **P < 0.01.