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. 2016 Jun 17;6:28452. doi: 10.1038/srep28452

Figure 5. GlcN effects on metabolic profiles.

Figure 5

(a) Metabolites of anabolic pathways supplied by glucose or glutamine. (b) Metabolites of energy production (TCA cycle or oxidative phosphorylation) fueled by glucose or glutamine. LG-ESC were grown in low glucose media ± 0.8 mM GlcN for 47 h, and then half of the dishes were replaced with the same media and half were replaced with 0 glucose media ± 0.8 mM GlcN (24 plates total, 6 per treatment group) for 1 additional hr of culture. Methods for metabolic profiling, metabolite identification, and data analysis are described in the Supplementary Methods. Statistical analysis of log-transformed data was performed using “R” (http://cran.r-project.org/). Data (mean ± s.e.m., N = 6) were analyzed by two-way ANOVA to determine main effects of glucose (Gluc), GlcN, and Gluc:GlcN interaction. Multiple comparisons were accounted for by estimating the false discovery rate (FDR) using q-values. *q < 0.05, **q < 0.01, ***q < 0.001, ****q < 0.0001. (q values of Gluc:GlcN interactions are shown next to vertical brackets.) Detailed quantitation and results of statistical analyses are shown in Supplementary Table 2.