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. 2016 Jun 17;6:28340. doi: 10.1038/srep28340

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Immunofluorescence staining of TM expression in RAW 264.7 cells, PBMCs and their differentiated osteoclast-like cells induced by treatment with M-CSF (20 ng/mL) and RANKL (30 ng/mL) for (A) 4 days and for (D) 1 week (original magnification × 200). Western blot analysis of TM and CATK expression in (B) RAW 264.7 cells and (E) PBMCs at indicated times after treatment with M-CSF and RANKL. Representative figures from three independent experiments are shown. (C,F) Quantitative representation of (B,E). Statistics were performed by Student’s t-test. **P < 0.01, ***P < 0.001.