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. 2016 Jun 17;6:28340. doi: 10.1038/srep28340

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(A) TRAP staining of osteoclasts differentiated from primary cultured TM^flox/flox and LysMcre/TM^flox/flox mice macrophages, treated with M-CSF and various concentrations of RANKL for differentiation (original magnification × 200). (B) Calculated ratio of TRAP⁺ MNCs to total cells per well. (C) Quantitation of TRAP activity using 4-NPP as substrate. Statistics were performed by Two-way ANOVA. *P < 0.05, ***P < 0.001. Data showed the mean ± SD for three independent experiments.