Figure 3. Inhibition of RANKL-induced osteoclast formation by TM lectin-like domain on macrophages.

(A) TRAP staining of osteoclasts differentiated from primary cultured TM^WT/LeD and TM^LeD/LeD mice macrophages, treated with M-CSF and various concentrations of RANKL for differentiation (original magnification × 200). (B) Ratio of TRAP⁺ MNCs to total cells per well. (C) Quantitation of TRAP activity using 4-NPP as substrate. Statistics were performed by Two-way ANOVA. **P < 0.01, ***P < 0.001. Experiments were repeated three times.