Table 1. GFP-encoding genes identified in cephalochordates.

Clade	Species	Gene name	Gene model ID	Chromophore region	Genomic coordinate (scaffold:start-end:strand)
GFPa	B. floridae	GFPa1	63256	HL GYG YY	Bf_V2_107:1004669–1007396:+
		GFPa2	63262	HL GYG YY	Bf_V2_107:912177–916945:+
GFPb	B. floridae	GFPb1	75522	HL GYA YY	Bf_V2_131:724134–735690:−
		GFPb2	75521	HL GYA FN	Bf_V2_131:710687–716998:−
		GFPb3	75519	QI GYG FH	Bf_V2_131:674613–680969:+
		GFPb4	75520	HI GYG FY	Bf_V2_131:693853–703767:+
	B. belcheri	GFPb1	233630F	HF GYG YD	scaffold58:854039–856682:+
		GFPb2	264830F	HF AYG YD	scaffold718:9844–13364:+
		GFPb3	240030F	HV GYG YH	scaffold6:4139317–4157041:+
GFPc	B. floridae	GFPc1	75523	NI GYG FH	Bf_V2_131:741722–750056:+
	B. belcheri	GFPc1	199320R	NL GYG FH	scaffold44:34684–36671:-
GFPd	B. floridae	GFPd1	86184	HL GYG HY	Bf_V2_226:1594162–1619868:−
		GFPd2	126982	HL GYG HY	Bf_V2_106:577911–582844:+
	B. belcheri	GFPd1	282900F	HL GYG FY	scaffold83:996645–1004389:+
		GFPd2	005130R	HL GYG FY	scaffold1:5804381–5807161:−
		GFPd3	005140R	HL GYG FY	scaffold1:5811232–5813910:−
		GFPd4	145360F	HL GYG FY	scaffold291:172581–179080:+
		GFPd5	233640F	HL GFG FY	scaffold58:860037–874989:+
GFPe	B. floridae	GFPe1	63257‡	NL GYG FY	Bf_V2_107:979539–982204:+
		GFPe2	63260	NL GYG FY	Bf_V2_107:950946–955643:+
		GFPe3	63258	NL GYG FY	Bf_V2_107:971176–973558:−
	B. belcheri	GFPe1	144690R	NL GYG FY	scaffold29:2454423–2458156:−
GFPf	B. floridae	GFPf1	63259	NL GYG YH	Bf_V2_107:961008–966261:−
	B. belcheri	GFPf1	144780R	NL GYG YH	scaffold29:2657919–2677300:−
	B. belcheri	GFPf2	212280R	NL GYG YH	scaffold5:926780–961355:−
	A. lucayanum	GFP1	asym20h*	NL GYG YH
Un- classified	B. belcheri	GFPx1	276530F	HL GYG FY	scaffold8:2499615–2549717:+
	A. lucayanum	GFP2	asym20h**	HL GYG LY

^‡The internal sequence gap within the B. floridae gene model 63257 was filled based on our previous study²³.

^*Asym20h_comp74545_c2_seq1_m.28460.

^**Asym20h_comp64813_c0_seq2_m.13123.