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| Km±s.e.m. (μM) | Vmax±s.e.m. (× 106) | |
|---|---|---|
| D-luci | 107±14.7 | 13.8±0.71 |
| CycLuc1 | 1.06±0.06 | 7.00±0.09 |
| Aka-HCl | 2.06±0.63 | 2.07±0.23 |
Aka-HCl, AkaLumine-HCl, CycLuc1, cyclic alkylaminoluciferin; D-luci, D-luciferin.
Km and Vmax values of D-luci, CycLuc1 and Aka-HCl. These values were calculated from reactions with recombinant Fluc protein and the substrate in the presence of Mg-ATP.
