FIG 1.

Basal expression of GADD34 mRNA and protein. (A) mRNA levels, translational efficiency, and total translation of GADD34 mRNA in WT MEFs were assessed by ribosome profiling and RNA-seq. (B) GADD34 protein levels in unstressed WT MEFs and increased levels in MEFs subjected to ER stress (1 μM Tg for 6 h) were detected by immunoblotting with anti-GADD34 antibody. Lysates from unstressed and stressed GADD34^−/− MEFs lacked the immunoreactive band. (C) Levels of eIF2α phosphorylation in WT and GADD34^−/− MEFs were detected by immunoblotting with anti-P-eIF2α antibody. Total levels of eIF2α used as a loading control are also shown. (D) Box plot indicating the percentage of change in basal eIF2α phosphorylation in GADD34^−/− MEFs relative to WT MEFs assessed by immunoblotting and quantification by ImageJ (n = 5).