FIG 1.

Sunitinib does not inhibit RCC cell proliferation or signaling. (A) Proliferation assays of the indicated RCC cell lines treated with vehicle (Ve) or sunitinib (Su; 1 μM). Data are means ± standard errors of the means (SEM); n = 4. RLU, relative luminescence units. (B and C) Western blot analysis of RCC cell lines treated with Ve, rapamycin (Ra; 25 nM), or Su (10 μM) overnight (ON) (B) or of the indicated tumor grafts in mice treated with Ve, Ra, or Su for 3 days (C). (D) Hematoxylin and eosin (H&E) staining and IHC analysis of the same tumors as those used for Western blotting in panel C (×100 magnification).