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. 2016 Jun 15;36(13):1836–1855. doi: 10.1128/MCB.00189-16

FIG 3.

FIG 3

786-O cells support EC survival in a VHL gene-dependent and sunitinib-sensitive manner. (A) Coculture assay schematic. (B) Histogram of EC numbers in cocultures with the indicated cell lines. #, EC number in cultures supplemented with recombinant VEGF (100 ng/ml). (C) Histogram of the effects of VHL gene reconstitution on EC numbers and VEGF levels using RCC3 (VHL gene deficient) or WT8 (VHL gene reconstituted) (n = 4). (D) Histogram of the effects of sunitinib (Su; 100 nM) on EC numbers and VEGF levels in cocultures of 786-O cells. Data are means ± SEM. *, P < 0.05; **, P < 0.01, ***, P < 0.001. In panel C, n = 21 for 786-O, n = 8 for RCC3, n = 13 for WT8, and n = 10 for HEK293; in panel D, n = 21 for EC numbers and n = 11 for VEGF levels.