FIG 8.

FGF2 restores EC proliferation and signaling despite the presence of sunitinib, but FGF2 production does not correlate with sunitinib resistance in RCC. (A) Western blot analysis of HUVEC or HDMEC pretreated with Ve or Su (100 nM) and stimulated with VEGF (50 ng/ml) or VEGF plus FGF2 (50 ng/ml). (B) Proliferation curves of HUVEC and HDMEC treated with Ve or Su in the absence or presence of VEGF (100 ng/ml) or FGF2 (50 ng/ml) or both. (C) Neutralizing assay using anti-FGF2 antibodies to suppress the effect of FGF2. Data are means ± SEM. n = 4 (for panels B and C). (D) FGF2 levels in coculture supernatant from sensitive and resistant RCC lines. Horizontal bars indicate medians. (E) Correlation between FGF2 levels and sunitinib sensitivity (R = 0.035 and P = 0.91).