. 2016 Jun 15;1(3):e00130-16. doi: 10.1128/mSphere.00130-16

TABLE 2 .

Recovery of unselected markers among Ade⁻ transformants^a

Experiment	Transformant selection	No. of red transformants by phenotype^b				Total no. of transformants
Experiment	Transformant selection	Nat^S	Nat^R	Arg⁻	Arg⁺	Total no. of transformants
1	Arg⁺	17	3	NA	20	20
2	Arg⁺	15	8	NA	23	23
3	Arg⁺	26	15	NA	41	41
3	Nat^R	NA	20	15	5	20

ade2Δ::ARG4 and ade2Δ::NAT deletion templates were mixed in a 1:1 molar ratio. BWP17 cells were transformed with the mixture along with the CaCAS9 and sgRNA ADE2.1 PCR products. Transformants were selected through either an Arg⁺ or Nat^R phenotype, as indicated in the table. Red transformants were purified and scored for the unselected marker. The results of three independent experiments are shown.

NA, not applicable because the selection did not allow recovery of the phenotype.