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. 2016 May 25;7(9):1049–1056. doi: 10.7150/jca.14778

Figure 1.

Figure 1

Immunohistochemical staining for CDK5 and p27 expression in gastric cancerous tissue and the criteria for immunohistochemistry scoring. (A) Intensity score for CDK5 expression in gastric cancer tissue. a1 to a4: no staining (score 0), weak staining (score 1), moderate staining (score 2) and strong staining (score 3). The protein expression was considered low if the score was 1 or less and high if it was 2 or more. Scale bar = 100μm. (B) Distribution and intensity score for p27 expression in gastric cancer tissue. b1 to b4 (distribution score): ≤ 5% positive cells (score 0), 6% to 25% positive cells (score 1), 26% to 50% positive cells (score 2) and ≥ 51% positive cells (score 3). Scale bar = 100μm. b5 to b8 (intensity score): no staining (score 0), weak staining (score 1), moderate staining (score 2) and strong staining (score 3). The protein expression was considered low if the total score (distribution score + intensity score) was 3 or less and high if it was 4 or more. Scale bar = 50μm.