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(A) PCa cell lines were cultured on Matrigel. PC3 and DU-145 (4×105cell/ml) were able to form vascular channels 4 hours after seeding, whereas LNCaP (3×105cell/ml) failed to form patterned networks even when cultured for up to 48 hours. (Original magnification ×100, bar: 40µm). (B) Expression of EphA2 in PCa cell lines. Both mRNA and protein levels of EphA2 in PC3 and DU-145 were significantly higher than in LNCaP. (*, P<0.05, **, P <0.001).
