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Analysis of VM formation after specific knockdown of EphA2 in PC3 and DU-145 cells (original magnification ×100, bar: 40µm). (A) Messenger RNA and protein levels of EphA2 were significantly decreased 48 hours after transfection. (B) siRNA-mediated down-regulation of EphA2 in PC3 and DU-145 cells significantly impaired VM formation on Matrigel in both PC3 and DU-145 cells.
