Fig 3.

Effect of YSY01A on regulatory proteins important for cell proliferation and anti-apoptosis in cisplatin-resistance cancer cells. (A) Expression levels of specific proteins that are involved in cell proliferation and anti-apoptosis were analyzed by Western blotting with antibodies indicated after SKOV3/DDP cells were exposed to 0 (vehicle control), 200 or 400 nM YSY01A. GAPDH was used as a loading control. (B) SKOV3/DDP cells were treated as indicated followed by extracting total RNA and real-time PCR analysis of selected genes. GAPDH was used as an internal control. (N=3; ** p<0.01, by Student's t-test as compared with vehicle control)