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. 2016 Jul;22(7):1055–1064. doi: 10.1261/rna.055137.115

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© 2016 Gentry et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Time course of ribosome assembly in cells overexpressing wild-type and R331A DbpA. (A) The time course of ribosome profiles of cells overexpressing wild-type DbpA. (B) Time course of ribosome profiles of cells overexpressing R331A DbpA. The absorbance readings are shown as gray circles and the CPM of ³H labeled RNA as blue circles. The ribosome profiles of both wild-type and R331A DbpA were performed at 0, 5, 10, 15, and 30 min after addition of rifampicin, which stops new RNA transcription. These data show that over time and with different observed microscopic rate constants (Table 3) the 27S, 35S, and 45S particles accumulating in the cells overexpressing R331A DbpA and 45S particle accumulating in the cells overexpressing wild-type DbpA form the 50S subunit.