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. 2016 Jul;22(7):979–994. doi: 10.1261/rna.055251.115

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© 2016 Wroblewska and Olejniczak; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Hfq accelerates the association of RybB, SdsR, and MicC sRNAs to ompD mRNA. The kinetics of annealing of ³²P-labeled ompD-187 (1 nM concentration) to (A) RybB, (B) SdsR, or (C) MicC sRNA at 25 nM concentration, in the absence or presence of 3 nM Hfq. Free ompD-187 is marked as O, ompD-187-Hfq complex as O-H, ompD-187-RybB complex as O-R, ompD-187-RybB-Hfq ternary complex as O-R-H, ompD-187-SdsR complex as O-S, ompD-187-SdsR-Hfq ternary complex as O-S-H, ompD-187-MicC complex as O-M, and ompD-187-MicC-Hfq ternary complex as O-M-H. The control reactions, in which ³²P-ompD-187 alone was bound to Hfq, were supplemented with 2 nM cold ompD-187 mRNA to obtain the total concentration of RNA equal to that of Hfq. (D) The data from A, B, and C were plotted versus time, and the fitting of data provided k_obs values, which are presented on the plot. The average k_obs values are shown in Table 2.