FIGURE 6.

The boundary experiments with 5′-³²P-labeled ompD-187 mRNA suggest that it contains a strong Hfq binding site in the 5′-untranslated region. The denaturing gel analysis of the binding of Hfq to the 5′-labeled ompD-187 degradation fragments obtained by partial digestion with RNase T1. The untreated ompD-187 sample was resolved in the lane marked C, the formamide ladder in lane OH, and reaction with RNase T1 in denaturing conditions in lane T1 D. The positions of G-specific cleavages by RNase T1 are indicated on the left side of the gel.