Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 1;30(11):1300–1312. doi: 10.1101/gad.279836.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Nemajerova et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 6. — TAp73 is necessary and sufficient to drive motile multiciliogenesis and functions upstream of FoxJ1 in the transcriptional network. Functional rescue. (A) MCC-specific TAp73α is necessary and sufficient to fully rescue ciliogenesis in p73 knockout airways. TAp73α reconstituted p73 knockout (p73KO) MCCs produced large numbers of long motile cilia indistinguishable from wild-type (WT) cilia. Wild-type and p73 knockout MTECs uninfected (Control) or infected at seeding with lentivirus targeting mouse TAp73α expression to MCC fated cells. Representative SIM images on ALI D14 stained for Ac α-tub. (B) Quantitation of phenotypic rescue in various percentages of fully ciliated cells at ALI D4 and D14. Data were derived from five wild-type and five p73 knockout mice. (C,D) Reintroduction of TAp73α induces expression of FoxJ1 in p73 knockout MTEC cultures to wild-type levels. Wild-type and p73 knockout MTECs uninfected (Control) or infected at seeding with lentivirus targeting mouse TAp73α expression to MCC fated cells. (C ) Representative confocal images on ALI D14 stained for FoxJ1. (D) Quantitation of FoxJ1-positive cells at ALI D4 and D14. Data were derived from five wild-type and five p73 knockout mice. (E–G) Epistasis analysis. p73 knockout MCCs are 100% rescued by FoxJ1. Wild-type and p73 knockout MTECs uninfected (Control) or infected at seeding with lentivirus targeting mouse FoxJ1 expression to MCC fated cells. (E) Representative SIM images on ALI D14 stained for Ac α-tub. (F ) Quantitation of rescue in various percentages of fully ciliated cells at ALI D4 and D14. (G) Representative SEM images of p73 knockout MTECs on ALI D14 uninfected or infected with FoxJ1. See also Supplemental Figure S8D for representative confocal images used for F.