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. 2016 Jun 1;30(11):1327–1338. doi: 10.1101/gad.280834.116

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© 2016 Wahba et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — PolyA tracts directly affect hybrid formation. (A) PolyA-associated hybrid region. Snapshot of a representative polyA-associated hybrid 5′ of LEU9 showing the hybrid signal from rnh1Δ rnh201Δ (top) and rnh1Δrnh201Δ treated with RNase H in vitro (bottom). (B) Schematic of a locus containing a polyA tract (polyA) and a locus with a polyA tract seamlessly deleted (pAΔ). The locations of DRIP-qPCR amplicons are indicated with black bars. (C) Hybrid signal measured by DRIP-qPCR at polyA-containing genes. Hybrid signal was determined in rnh1Δrnh201Δ strains containing polyA tracts (solid bars) and deleted for the polyA (hatched bars) at the indicated genes. (D) Model of hybrid-promoting sequence features in yeast.