Table 2.

Description of the most useful tools to investigate redox capacity in vivo and in vitro

Method	Read-out	Detection	advantage/ limitation	References
OxICAT	Reduced peptides: 13C Oxidized peptides: 12C	Thiol groups	Identification of oxidative cysteines/ does not distinguish between compartments	16,91,98,111,117
roGFP	Excitation: 405/488 nm Emission: 509 nm	S-S bonds	Ratiometric measurements	15,97,102,103
HyPer	Excitation: 420/500 nm Emission: 516 nm	Hydrogen peroxide	Ratiometric measurements/pH sensitivity	104-108,116
Amplex UltraRed	Excitation: 544 nm Emission: 590 nm	Peroxide	Quantification of endogenous peroxide/ pH sensitivity	16,109,110,115
Killer-Red	Excitation: 585 nm Emission: 610 nm	Peroxide	Temporal and spatial ROS production / phototoxicity	112
SuperNova	Excitation: 579 nm Emission: 610 nm	Peroxide	Temporal and spatial ROS production / phototoxicity	113
miniSOG	Excitation: 448 nm Emission: 500/528 nm	Singlet oxygen	Temporal and spatial ROS production / phototoxicity	114