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. Author manuscript; available in PMC: 2016 Jun 17.
Published in final edited form as: J Proteome Res. 2015 Apr 29;14(6):2511–2519. doi: 10.1021/acs.jproteome.5b00076

Figure 1. Workflow outlining tissue sample preparation for combined lipidomics and MALDI-IMS.

Figure 1

(1) Serial coronal sections from GSC xenografts at 10 and 15 μm. (2) MALDI-IMS (10 μm) and lipid extraction ESI-MS/MS (15 μm) experiments were performed in parallel. (3) The identity of biological species of interest was confirmed by MS/MS. Lipid extraction and ESI-MS/MS provide quantitative measures of lipid species at high-resolution with submillimass accuracy and MALDI-IMS provides lipid localization of identified lipids as intact molecular ions.