Fig 2.

A) Viability of S. cerevisiae culture during freezing-thawing challenge in the presence of 3HB applied at 50 mM and 100 mM and in its absence B) Viability of the yeast culture exposed to repeated freezing-thawing in the presence of 3HB, trehalose and glycerol (all protectants were applied at 100 mM concentration) and in absence of a cryoprotectant. *note: Statistical significance was tested using 2 sample t-test (Minitab), each sample was compared with control, all the differences were found statistically significant.