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. 2016 Jun 17;11(6):e0157778. doi: 10.1371/journal.pone.0157778

Fig 3.

Fig 3

A) Viability of PHB-accumulating C. necator H16 and PHB non-accumulating C. necator PHB-4 exposed to repeated freezing-thawing challenge in the presence and absence of 100 mM 3HB. B) Viability of C. necator H16 and C. necator PHB-4 exposed to various sub-zero temperatures without addition of 3HB. *note: Statistical significance was tested using 2 sample t-test (Minitab). Presence of 3HB resulted in statistically significant increase in viability for both tested cultures and for all freezing-thawing cycles. Difference between viability of C. necator H16 and C. necator PHB–4 was statistically significant for all experimental conditions except for the initial 2 freezing-thawing cycles in absence of 3HB. In Fig 3B viability of C. necator H16 was compared with viability of C. necator PHB-4 for each temperature of incubation; statistically significant differences are labeled by asterisks.