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. 2016 Jun 17;12(6):e1005703. doi: 10.1371/journal.ppat.1005703

Fig 3. Appressorium development and chitin deacetylation are dramatically reduced in the CDA deletion strains.

Fig 3

A) Table showing percentage conidial germination (at 1 hpi) and percentage of germ tubes forming appressoria (at 8 and 24 hpi) on a hydrophobic glass surface (± SD, n = 3). Germination rates were analyzed using Welch's ANOVA, with Games-Howell post-hoc test. Means not sharing a letter indicate significant differences (p < 0.05). Significantly reduced appressorium formation frequency was observed in certain deletion strains (1-way ANOVA with post-hoc Tukey test. Means not sharing a letter indicate significant differences p < 0.05). ‡: data could not be analyzed using ANOVA, as did not fulfil requirements. B) Fluorescent labelling of chitosan on germlings of the CDA deletion strains at 16 hpi, showing complete lack of staining in the cda2/cbp1 and cda2/cbp1/cda3 strains. Scale bars: 20 μm.