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. 2016 Jun 17;11(6):e0157663. doi: 10.1371/journal.pone.0157663

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© 2016 Konrat et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (a) SEM image: overview on a glass bead after 24 h cultivation with P. aeruginosa. (b) The bead surface is evenly covered with biofilm. (c) The bacteria are densely arranged in a monolayer. (d) Overview on a glass bead after the biofilm had been removed by sonication. (e, f) The bead surface is virtually empty, except for some residual debris. (g) CLSM image: The sugar-matrix of the P. aeruginosa biofilm was stained with Concanavalin A (assigned color: magenta), and the bacteria with Syto60 (assigned color: green). (h) LIVE/DEAD^® staining of the biofilm on a glass bead. (i) LIVE/DEAD^® staining after the biofilm had been removed from the bead by sonication.